探讨外周血干细胞采集过程中最佳补钙方法

Study on the best method of calcium supplementation during peripheral blood stem cell harvesting

目的:探寻预防和治疗外周血干细胞(PBSC)采集过程中低钙血症的最佳补钙方法。方法:对175例正常健康供者行222例次PBSC采集,按补钙方法不同将222例次采集分为口服组(60例次),静脉推注组(71例次)和静脉滴注组(91例次)。观察采集过程中供者低钙血症的症状,并检测采集物中有核细胞和CD34+细胞的量。结果:口服组低钙血症发生率及严重程度均明显高于静脉推注组和静脉滴注组(P<0.05)。而静脉推注组与静脉滴注组在低钙血症发生率和严重程度方面差异无统计学意义(P>0.05)。3组采集物中有核细胞数和CD34+细胞数差异均无统计学意义(P>0.05)。结论:静脉滴注补钙方法预防和治疗PBSC采集过程中的低钙血症效果好,操作方便,且不影响采集效果。

Objective： To explore the best way for prophylaxis and treatment of hypocalcemia during peripheral blood stem cell（PBSC）harvesting. Methods： 175 healthy donors were underwent 222 collections.According to the different methods of administration of calcium gluconate,all the 222 procedures of PBSC harvesting were divided into group oral administration（60cases）,group intravenous injection（71cases） and group intravenous drip（91cases）.Hypocalcemia associated symptoms were observed during collection,and,nucleated cell and CD34＋ cell counting in samples from collection bag was performed. Results： Both the incidence and degree of hypocalcemia in group oral administration were higher and graver than the other two groups（P〈0.05）.But,there was no significant statistical difference between group intravenous injection and group intravenous drip in the incidence and degree of hypocalcemia（P〉0.05）.No significant differences were found among the three groups in terms of mean number of nucleated cells and CD34＋ cells（P〉0.05）. Conclusion： Intravenous drip of calcium gluconate is an effective method for prophylaxis and treatment of hypocalcemia during PBSC harvesting,which is simple and convenient for carrying out,while the efficiency of harvesting is not affected.