血管紧张素Ⅱ1型受体在人胃癌细胞株中的表达及血管紧张素Ⅱ对MKN-28细胞增殖和周期的影响

Expression of angiotensin Ⅱ type 1 receptor(AT1R) in human gastric cancer cell lines and effects of angiotensin Ⅱon MKN-28 cell proliferation and cell cycle

目的：探讨血管紧张素Ⅱ（angiotensinⅡ,AngⅡ）1型受体（angiotensinⅡtype 1 receptor,AT1R）在人胃癌细胞株中的表达及AngⅡ对AT1R高表达胃癌细胞（MKN-28）增殖和周期的影响。方法 ：采用Western印迹法检测AT1R在胃癌细胞中的表达。应用不同浓度的AngⅡ（10-10~10-5mol/L）、AT1R阻滞剂（氯沙坦）以及AngⅡ2型受体（angiotensinⅡtype 2 receptor,AT2R）阻滞剂（PD123319）作用于MKN-28细胞,以CCK-8法测定各处理组细胞的相对数目,计算增殖促进效应;应用流式细胞仪检测各处理组细胞周期变化,计算各处理因素对细胞周期的影响。结果：AT1R在多数胃癌细胞株中表达,其中MKN-28细胞株表达量最高,AngⅡ可明显促进MKN-28细胞增殖以及G1期细胞向S、G2期转化,氯沙坦可明显抑制AngⅡ对MKN-28细胞的促增殖及促进G1期细胞向S、G2期转化的作用,PD123319无此作用。结论：AngⅡ通过AT1R明显促进MKN-28细胞增殖,促进G1期细胞向S、G2期转化。

Objective To investigate the expression of angiotensin Ⅱ type 1 receptor（AT1R） in human gastric cancer cell lines,and the effect of angiotensin Ⅱ（AngⅡ） on MKN-28 cell proliferation and cell cycle.Methods The AT1R expression in human gastric cancer cell lines was detected by Western blot.The MKN-28 cells were treated with different concentrations of Ang Ⅱ（10-10 - 10-5 mol/L）,AT1R blocker（losartan）,and Ang Ⅱ type 2 receptor（AT2R） blocker（PD123319）.Cell proliferation was measured by cell counting kit-8 and cell cycles in different treated groups were measured by flow cytometry.Results Most of the human gastric cancer cell lines expressed AT1R.In these cell lines,MKN-28 cells had the highest level of AT1R expression.AngⅡ significantly enhanced the MKN-28 cell proliferation and promote the transformation from G1 phase to S,G2 phase in MKN-28 cell line.All of these effects could be inhibited by losartan,but not PD123319.Conclusions AngⅡ can significantly promote the MKN-28 cell proliferation and accelerate the cell cycle transformation from G1 phase to S,G2 phase via AT1R.