金银花活性成分提取及其抗氧化和抑菌功效研究

Extraction of active ingredients in Honey suckle and their function in cosmetics

利用超声波预处理结合乙醇回流提取法从金银花中提取活性成分,通过响应面分析法设计实验,得到了较佳的提取工艺:超声波预处理30.9 min,乙醇的体积分数为73.7%,提取温度为68.5℃,每次提取2.1 h,提取2次,在此条件下绿原酸提取率达到88.39%。通过与阳性物质VC比较检验了提取物的功效,证明金银花提取物具有较好的体外抗氧化能力;通过对大肠杆菌、金黄色葡萄球菌、黑曲霉的抑制分析,证明金银花提取物具有良好的抑菌防腐能力。

Active ingredients in Honey suckle were extracted by ultrasonic-aided ethanol method.The optimal processing conditions for the extraction were identified by using the software ＂Design Expert 7.1.3 Tril＂.Pretreatment time is 30.9 min under aid of ultrasonic wave.Ethanol with volume fraction of 73.7% was used as the solvent.Temperature was 68.5 ℃.Extraction of two times was operated with each of 2.1 h.Under the afore-said conditions,yield of chlorogenic acid achieves 88.39%.Comparing with positive material vitamin C,the efficacy of the extract was investigated and experimental results showed that the extract has a good in-vitro antioxidation capability.Bacteriostasis tests were carried out against colibacillus,Staphylococcus aureus and Aspergillus niger and the results showed that the Honey suckle extract has strong bacteriostatic and antiseptic capabilities.