快速溶剂萃取提取山豆根中苦参碱和氧化苦参碱最佳条件研究

Optimization of Extraction of Matrine and Oxymatrine from Radix Sophorae Tonkinensis by Accelerated Solvent Extraction

目的研究山豆根中苦参碱和氧化苦参碱的最佳提取工艺。方法以苦参碱和氧化苦参碱的总含量为考察指标,利用高效液相色谱法测定其含量,比较了温浸、超声、回流和快速溶剂萃取(ASE)4种提取方法对山豆根苦参碱和氧化苦参碱提取效果的影响,通过单因素和正交实验优化ASE的提取条件。结果 ASE在提取效果上优于其他3种提取方法,影响山豆根苦参碱和氧化苦参碱提取效果的各因素主次顺序为:提取次数(D)>乙醇体积分数(A)>提取温度(B)>提取时间(C),ASE的最佳提取工艺:乙醇体积分数为40%,提取温度为90℃,提取时间13 min,提取2次。在该条件下,得出苦参碱和氧化苦参碱总含量为(15.527 7±0.431 6)mg.g-1。结论该实验优选出山豆根生物碱的最佳提取方法,并优化了其参数,为山豆根生物碱的提取提供了一种新的方法。

OBJECTIVE To study the optimal extraction technology of matrine and oxymatrine from Radix Sophorae Tonkinensis. METHODS The total content of matrine and oxymatrine as the inspection index was determined using high performance liquid chromatography （HPLC）. The extraction efficiency of warm immersion extraction, ultrasonic extraction, reflux extraction and accelerated solvent extraction （ASE） was compared. RESULTS Accelerated solvent extraction was superior to the other three methods. The extraction conditions of accelerated solvent extraction were optimized through single-factor test and orthogonal experimental design. The sequence of in- fluential significance was as follows ： extraction times 〉 ethanol concentration 〉 extraction temperature 〉 extraction time. The optimal technology of accelerated solvent extraction was that the ethanol concentration was 40% ,extraction temperature was 90 ℃ ,the time of extraction was 13 rain and the times of extraction was twice. The total content of matrine and oxymatrinc from Radix sophorac tonkinensis was （ 15.527 7 ± 0. 431 6） mg . g-1 CONCLUSION The experiment has optimized the extraction method and parameters of alkaloid from Radix sophorae tonkinensis, which provides a new method for the extraction of alkaloid from Radix sophorae tonkinensis.