红芪多糖3中4个组分的单糖组成分析及多糖含量测定

Analysis of the Composition of Monosaccharides and the Contents of Polysaccharides in Four Components of Radix Hedysari Polysaccharide 3

目的对红芪多糖3(HPS-3)进行分离纯化,研究红芪多糖3中4个组分单糖组成以及多糖含量测定。方法红芪多糖经水提醇沉,Sevag法脱蛋白,H2O2脱色,醇沉,透析,再经DEAE-cellulose 52和Sephadex G-100柱色谱纯化,得到HPS-3-A、HPS-3-B、HPS-3-C、HPS-3-D 4个组分;HPGPC法分析,4个组分均为单一组分;TLC法与GC法分析4个组分单糖组成;改良苯酚硫酸法测定4个组分中多糖含量。结果 HPS-3-A主要由葡萄糖组成,多糖含量为99.2%;而HPS-3-B、HPS-3-C、HPS-3-D主要由阿拉伯糖与半乳糖组成。多糖含量依次为96.5%,95.3%,95.2%。结论本实验为红芪多糖3构效关系研究提供基础。

OBJECTIVE To isolate and purify radix hedysari polysaccharide 3 （ HPS-3 ） and determine the monosaccharide composition and the contents of polysaccharide in its four components. METHODS Radix Hedysari polysaccharide was extracted by water,precipitated with ethanol, treated with Sevag method, decolored with H2O2, then precipitated with ethanol and dialyzed. By using DEAE-cellulose 52 and Sephadex G-100, HPS-3-A, HPS-3-B, HPS-3-C and HPS-3-D were purified. Their purity were determined by high performance gel permeation chromatography （HPGPC）. By TLC and GC, the monosaccharide composition of four components was analyzed, and then the modified phenol sulfuric acid method was used to determine the content of polysaccharide of the four fractions. RESULTS HPS-3-A was composed mainly of glucose, and the content of polysaccharide was 99. 2%. But HPS-3-B, HPS-3-C and HPS-3-D were all composed mainly of arabinose and galactose. The contents of polysaccharide of them were 96. 5% , 95.3% and 95.2%, respectively. CONCLUSION This study provides the foundation for the research of structure-function relationship of Radix Hedysari polysaccharide 3.