大环内酯类和氟喹诺酮类药物对细胞内嗜肺军团菌的效果分析

Activity of macrolides and fluoroquinolones against intracellular Legionella pneumophila

目的分析了大环内酯类和氟喹诺酮类药物在抑制巨噬细胞内嗜肺军团菌的疗效。方法选取10株不同嗜肺军团菌血清1型,利用琼脂稀释法对不同嗜肺军团菌血清1型针对大环内酯类和氟喹诺酮类药物的半致死浓度进行分析。THP-1人单核细胞在佛波酯诱导下形成巨噬细胞,然后将不同嗜肺军团菌血清1型与巨噬细胞共同培养,待细胞将巨噬细胞吞噬后,采用半致死浓度不同倍数(1倍、4倍,8倍)的红霉素、阿奇霉素、左氧氟沙星及莫西沙星等药物对胞内的细菌进行抑制,共同培养24 h后,采用平板计数法对军团数量进行计算,用细菌抑制率进行表示。结果红霉素、阿奇霉素、左氧氟沙星及莫西沙星在半致死剂量、4倍半致死剂量、8倍半致死剂量下对细菌的抑制情况如下:红霉素:(51.42±23.19)%、(75.28±19.08)%及(90.52±9.87)%;阿奇霉素:(60.57±23.14)%,(90.25±9.68)%及(98.58±3.89)%;左氧氟沙星:(99.24±0.13)%、(99.56±0.58)%及(99.99±0.01)%;莫西沙星:(99.14±0.25)%、(99.36±0.35)%及(99.89±0.11)%。在不同半致死剂量下氟喹诺酮类药物对巨噬细胞胞内细菌的抑制率明显高于大环内酯类药物,具有统计学意义(P<0.05)。左氧氟沙星和莫西沙星在不同倍数的半致死剂量下对细菌的抑制率没有显著性差异P>0.05),阿奇霉素对巨噬细胞内细菌的抑制率显著性高于于红霉素,具有显著统计学意义(P<0.05)。结论氟唾诺酮类药物抑制胞内抗军团菌效果好于大环内酯类药物,左氧氟沙星和莫西沙星抑制效果相当,阿奇霉素抑制效果明显优于红霉素。

Objective To explore the inhibition activity of macrolides and fluoroquinolones against Legionella pneumophila in macrophage.Methods Minimum inhibitory concentration was determined by standard agar dilution.For intracellular assays,human monocytic cell line THP-1 were infected by legionella pneumonia.Erythromycin,azithromycin,levofloxacin and moxifloxacin at the different concentration were added,percentage inhibition was determined.Results Percentage inhibition were as follows： Erythromycin（51.42±23.19）%,（75.28±19.08）% and（90.52±9.87）%;Azithromycin（60.57±23.14）%,（90.25±9.68）% and（98.58±3.89）%;Levofloxacin（99.24±0.13）%,（99.56±0.58）% and（99.99±0.01）%;Moxifloxacin（99.14±0.25）%,（99.36±0.35）% and（99.89±0.11）%.Comparison macrolides,the fluoroquinolones at the different concentration showed greater inhibitory activity（P0.05）.Levofloxacin and moxifloxacin showed the same inhibitory activity（P0.05）.Comparison erythromycin,Azithromycin was more effective in inhibiting intracellular legionella pneumophila（P0.05）.Conclusions The fluoroquinolones showed higher activity against legionella pneumophila than macrolides.There were the same activity by levofloxacin and moxifloxacin against legionella pneumophila.Azithromycin have superior activity against legionella pneumophila than erythromycin.