大黄素对大鼠肝纤维化形成的影响

Effect of Emodin on Development of Hepatic Fibrosis in Rats

研究大黄素对肝纤维化的影响。方法：采用 ４０％的四氯化碳（ ＣＣｌ４）给予大鼠皮下注射诱导肝纤维化，并以小、中和大剂量大黄素（２０、４０和 ８０ｍｇ／ｋｇ体重）干预，测定血清透明质酸、层粘连蛋白及肝组织羟脯氨酸，并通过光镜和电镜观察肝组织病理变化。结果：大黄素组较模型组：（１）血清透明质酸及层粘连蛋白显著降低；（２）肝组织胶原蛋白含量明显减少；（３）肝组织纤维化程度明显改善；（４）肝细胞损伤减轻。结论：大黄素对大鼠肝纤维化具有治疗作用。

To investigate the effect of emodin on the development of hepatic fibrosis in rats. Methods: Forty percent of carbon tetrachloride (CCl4) dissolved in olive oil was injected subcutaneously to rats twice a week, for 6 weeks to produce hepatic fibrosis model, the rats of treated group were simultaneously injected with CCl4, treated with emodin in low, medium and high dose (20, 40 and 80mg/kg body weight respectively) once a day for 42 days, which was dissolved in 0.5% sodium carboxymethylcellulose (CMC), while for rats of the control group, they only received CCl4. and CMC. Changes of serum hyaluronic acid, laminin and liver hydroxyproline were determined and histopathologic changes of liver was examined by optical microscopy and electron microscopy. Results: Compared with the model group, in the emodin treated group: (1) Serum hyaluronic acid and laminin were markedly reduced (P<0.05or 0. 01); (2f) liver hydroxyproline was significantly decreased (P<0.05 or 0.01); (3) degree of fibrosis was reduced (P<0.0g5 or 0.01); (4) hepatocyte injury was ameliorated. Conclusion: Emodin has therapeutic effect on the development of hepatic fibrosis in rats. The effect might be related to slowing hepatocyte injury.