解偶联蛋白2基因Ala55Val变异与中国人脂代谢、体脂含量及分布的关系

The association between A55V variant in UCP2 gene and body fat distribution,serum lipid profile in Chinese

目的 探讨解偶联蛋白 2 (uncoupling protein2 ,UCP2 )基因 Ala5 5 Val(A5 5 V)变异与中国人脂代谢、体脂含量及分布的关系。方法 用 PCR- RFL P检测 35 9例无亲缘关系的中国人 [据 1997年 ADA标准分为 :糖耐量正常者 (normal glucose tolerance,NGT) 193例 ,2型糖尿病 16 6例 ]UCP2基因 A5 5 V变异的基因型 ,局部体脂分布参数用核磁共振仪 (magnetic resonance imaging,MRI)测定。结果 在 NGT组中 ,UCP2基因 A5 5 V变异与女性的体重指数 (body mass index,BMI)及股部皮下脂肪面积 (femoralsubcutaneous adipose tissue area,FA)相关 (P值分别为 0 .0 2 46及 0 .0 0 17) ;与男性的血甘油三酯(triglyceride,TG)水平相关 (P=0 .0 0 72 )。在 2型糖尿病组中 ,同样可以发现 UCP2基因 A5 5 V变异与女性的 FA相关 (P=0 .0 15 0 )。AA纯合子的女性 (无论是 NGT还是 2型糖尿病 )具有较低的 FA。L ogistic回归分析发现 :UCP2基因 A5 5 V变异与 FA(NGT女性 :P=0 .0 0 98;2型糖尿病女性 :P=0 .0 0 71)、BMI(NGT女性 :P=0 .0 0 16 )以及 TG水平 (NGT男性 :P=0 .0 0 40 )相关。结论 U CP2基因 A5 5 V变异与中国女性的 FA(无论是在 NGT还是在 2型糖尿病组中 )及 BMI(在 NGT组中 )相关 ,因此 UCP2基因该变异参与了?

Objective Uncoupling protein 2(UCP2) could play an important role in energy metabolism and body weight regulation. The aim of this study was to investigate Ala55Val(A55V) variant in the UCP2 gene has effects on serum lipid profile, body fat and its distribution in Chinese.Methods The genotypes of A55V variant in the UCP2 gene were determined by a PCR RFLP assay in 359 unrelated Chinese [including 193 normal glucose tolerance(NGT) and 166 type 2 diabetic subjects by ADA 97' criteria].The parameters for regional adipose tissue distribution were measured by magnetic resonance imaging(MRI). Results In NGT group, an association between A55V variant in the UCP2 gene and body mass index (BMI) (P=0.0246),as well as femoral subcutaneous adipose tissue area (FA) (P=0.0017), was noted in females. A55V variant in the UCP2 gene was also associated with serum triglyceride (TG) level (P=0.0072) in males. However, in type 2 diabetes group, an association between A55V variant in the UCP2 gene and FA (P=0.0150) was replicated in females too. Those females who were homozygotes of AA in the UCP2 gene had decreased FA not only in NGT group but also in type 2 diabetes group. Furthermore, logistic regression analysis indicated that FA (P=0.0098 in NGT females, P=0.0071 in type 2 diabetic females) and BMI (P=0 0016 in NGT females), as well as TG level(P=0.0040 in NGT males) were associated with this variant in the UCP2 gene.Conclusion A55V variant in the UCP2 gene is associated not only with FA(in NGT females and type 2 diabetic females) but also with BMI (in NGT females). Therefore A55V variant in the UCP2 gene appears to play a role in body fat and its distribution in Chinese females.