糖尿病性阴茎勃起功能障碍大鼠阴茎海绵体一氧化氮合酶活性的测定

Nitric oxide synthase activity in penile cavernous tissue of erectile dysfunction rat models with diabetes me-llitus

目的 探讨糖尿病 (DM )性阴茎勃起功能障碍 (ED)的发病机理。 方法 SD大鼠注射链脲佐菌素建立DM动物模型后 ,注射阿朴吗啡观察 6周、8周及 12周大鼠阻茎勃起情况 ,筛选DM性ED大鼠模型 ,测定其阴茎海绵体组织一氧化氮合酶 (NOS)的活性。 结果 DM性ED大鼠阴茎海绵体组织NOS活性与对照组相比显著降低 (P <0 0 0 1或P <0 0 1) ,随DM病程延长 ,NOS活性明显下降 (P <0 0 1)。 结论 DM严重影响阴茎勃起功能 ,海绵体组织NOS活性降低可能是其发病机理之一。

Objective To investigate the mechanism of erectile dysfunction (ED) in rat models with diabetes mellitus (DM). Methods Sprague Dewley rats were injected intraperitoneally with streptozotocin (STZ) to make experimental models of DM.Injected with apomorphine,penile erection of the rat models with DM and the controls were observed and noted on the 6th,8th and 12th week from the 4th day of STZ injection.NOS activity was examined in selected rat models. Results Compared with the controls,NOS activity in the penile cavernous tissue of ED rats with DM decreased significantly ( P <0 001 or P <0 01) and also decreased significantly( P <0 01) with the increased duration of DM. Conclusions Penile erection is seriously affected in rats with DM and the decrease of NOS activity might be one of the most important factors.