含IgG类抗Sm抗体对HEp2细胞增殖周期的影响

Influence of Anti Sm Antibody IgG on the Cell Cycles of Proliferation in HEp 2 Cells

本文研究进入性含IgG类抗 Sm抗体对体外培养的HEp 2细胞的影响。结果表明 :经SLE患者IgG (含抗 Sm )作用后 ,HEp 2细胞G0 /G1期减少 ,G2 /M期升高 ,Tc明显延长 ,部分细胞发生凋亡。从时间关系上看 ,至 2 16h左右 ,HEp 2细胞周期复原 ,凋亡率的下降与HEp 2细胞偏离正常的程度呈正相关。提示进入性抗 Sm在SLE病损中的作用可能是诱发受侵细胞凋亡。

The influence of anti Sm antibody IgG penetrating into HEp 2 cells on the cell cycles of cultures in vitro was determined Results showed that pretreatment of cells with sera of patients with SLE(containing the anti Sm) could induce the shortening of G 0/G 1 phases,prolongation of G 2/M phases,marked prolongation of Tc times and apoptosis of small proportions of cells The changes in cell cycles returned to normal in about 216 hrs,and the reduction of apoptotic rates positively correlated with the extent of deviation to normal in HEp 2 cells These results suggest that the action of penetrating anti Sm antibody in sera of patients with SLE might be due to the induction of apoptosis in affected cells