乙型肝炎表面抗原弱阳性样本的三种测定方法比较

Comparison of three methods to determinate hepatitis B surface antigen with weakly positive samples

目的比较胶体金免疫层析试验(GICA)和酶联免疫吸附试验(ELISA)与微粒子化学发光检测技术(MEIA)检测乙型肝炎表面抗原(HBsAg)弱阳性样本的结果符合率,以了解三种方法的特异性与灵敏度等诊断指标。方法用三种方法平行检测收集的血清标本HBsAg,并相互比较。结果 ELASA与MEIA结果符合率为94.9%;GICA与MEIA结果符合率为76.9%.GICA检测HBsAg弱阳性样本时的各项诊断指标都不甚理想。结论①GICA的灵敏度和特异性分别为81.3%和81.3%,较ELISA和MEIA低,检测HBsAg弱阳性样本时存在一定程度的漏检率和误诊率,只能起筛查作用。遇到HBsAg弱阳性的血清标本必须与ELISA法并用,实行双检;②ELISA较MEIA灵敏度和特异性略低,当测定标本的OD值在临界值(cut-off)附近即检测灰区时建议用MEIA复检。

Objectives To compare GICA and ELISA with MEIA detects low concentration HbsAg specimen to match a rate as a result,with understanding three kinds of method of specificity and sensitivity.Methods Use the serum specimen of the detection collections of three kinds of method parallelism HbsAg,and mutually compare.Results Three kinds of method detection HbsAg match the rate as 74.3% as a result,Among them,ELASA and MEIA match the rate as 94.9% as a result GICA and MEIA match the rate as 76.9% as a result.GICA detect low concentration HbsAg spcimen various diagnosis index signs not all really perfect.Conclusion ①To compare with ELISA and MEIA sensitive and specificity is 81.3% and 81.3%,the GICA is low.Use GICA detection low concentration HbsAg spcimen exist certain degree to leak check rate and misdiagnosis rate,GICA can sieve HbsAg speimen.Meet low concentration HbsAg spcimen have to combine with ELISA method,practicing a pair of check.②To compare with degree and specificity of the MEIA sensitivity,the ELISA was slightly low.When the OD measuresed specimen was worth in the cutoff neighborhood,to examine ash area namely the suggestion replies check with the MEIA.