再生障碍性贫血患者Fas抗原的表达及血浆可溶性Fas、FasL测定的临床意义

The expression of Fas antigen and plasma levels of sFas and sFasL in patients with aplastic anemia

目的 探讨Fas(CD95) /FasL(CD95L)系统在再生障碍性贫血 (再障 )中的表达及临床意义。方法 用酶联免疫夹心法检测 32例再障患者血浆可溶性Fas、FasL(sFas、sFasL) ,用流式细胞术检测骨髓CD3 4+ 细胞、CD3 4-细胞和外周血单个核细胞 (PBMNCs)Fas抗原的表达。结果 再障患者骨髓CD3 4+细胞Fas表达率显著高于正常对照 (P <0 .0 5 ) ,且重型再障组较慢性再障组显著增高 (P <0 0 5 ) ,重型再障和慢性再障CD3 4+ 细胞Fas表达均与病程呈负相关 ;再障患者血浆sFas显著低于正常对照 (P <0 .0 5 ) ,重型再障较慢性再障显著降低 (P <0 .0 5 ) ,血浆sFas与CD3 4+ 细胞Fas表达率呈负相关。结论 Fas/FasL系统表达异常与再障的发生、发展相关。

Objective To investigate the role of Fas/FasL system in the pathogenesis of aplastic anemia (AA) and its correlation with clinical status. Methods Plasma levels of sFas and sFasL and the expression of Fas antigen on mononuclear cell (MNC) membrane in 32 AA patients and 24 normal controls were assayed by ELISA and FACS,respectively. Results The percentage of CD 34 +Fas + cells was significantly higher(P<0.05) in AA patients than that in normal controls,and the percentage of CD 34 +Fas + cells in severe AA patients was much higher than that in chronic AA patients(P<0.05). The percentage of CD 34 +Fas + cells was negatively correlated with the course of disease. The levels of sFas were significantly lower (P<0.05) in AA patients than that in normal controls,and the sFas levels in severe AA were much lower than those in chronic AA (P<0.05). The percentage of CD 34 +Fas + cells was negatively correlated with sFas level. Conclusion Fas/FasL system aberration is involved in the pathogenesis of AA.