异氟醚对老年大鼠学习记忆的影响

Effects of Isoflurane on Learning and Memory in Aged Rats

目的探讨异氟醚吸入麻醉对(SD)老年大鼠学习和记忆的影响。方法 36只SD健康雄性大鼠随机分为异氟醚麻醉组和对照组,每组各18只。麻醉组给予2%异氟醚和40%氧气诱导及维持麻醉3 h,对照组单纯吸入含40%氧气的空氧混合气体3 h。麻醉组或对照组干预结束24 h后开始为期1周的盲法测试学习记忆能力。结果麻醉组大鼠与对照组大鼠相比,Y-迷宫测试成绩错误反应次数差异无统计学意义(P>0.05);除第7天麻醉组大鼠起步电压高于对照组(P<0.05)外,其余时间两组大鼠起步电压差异均无统计学意义(P>0.05);除第3天麻醉组大鼠跑步电压高于对照组(P<0.05)外,其余时间两组大鼠跑步电压差异均无统计学意义(P>0.05)。结论 2%单纯异氟醚吸入麻醉不改变老年大鼠麻醉后学习和记忆能力。

Objective To investigate the role of isoflurane inhalation on learning and memory in aged Sprague-Dawley（SD） rats. Methods A total of 36 SD rats were randomly assigned to control group and anesthesia group with 18 rats in each group. Rats in the anesthesia group were given 2% isoflurane for the purpose of anesthesia which was induced by 40% oxygen and last for 3 hours. Rats in the control group inhaled the mixture of air and 40% oxygen for 3 hours.Learning and memory abilities of these rats were assessed daily in Y-maze 24 hours after the anesthesia intervention.Results There were no statistically significant differences between the two groups in numbers of wrong reaction （P 〉 0.05）.The voltage when rats raised their feet was not significantly different between the two groups except that on the 7th day, it was higher in the anesthesia group than in the control group （P 〈 0.05）. The voltage when rat began to run was also not ignificantly different between the two groups except that on the 3rd day it was higher in the anesthesia group than in the control group （P 〈 0.05）. Conclusion Two percent isoflurane anesthesia could be of no effect on postanesthetic learning and memory in aged SD rats.