西安地区耐甲氧西林金黄色葡萄球菌的SCCmec基因分型分析

Analysis of SCCmec genotype in methicillin-resistant Staphylococcus aureus in Xi′an

目的:了解西安地区耐甲氧西林金黄色葡萄球菌(MRSA)的SCCmec基因型别。方法:应用聚合酶链反应(PCR)技术检测mecA基因并对其进行分型。结果:147株MRSA的mecA基因全部阳性,SCCmecⅡ型1株,SCCmecⅣa型1株,SCCmecⅢ型144株,还有1株用本实验方法未能分型。结论:SCCmecⅢ型为西安地区主要流行类型。

Objective： To investigate the SCCmec genotype in methicillin-resistant Staphylococcus aureus in Xi′an.Methods： Polymerase chain reaction（PCR） was used to determine the genotype of staphylococcal cassette chromosome mec（SCCmec）.Results： All MRSA isolates were mecA positive.Among the 147 isolates of MRSA,1 isolate belonged to SCCmecⅡ,1 isolate belonged to SCCmecⅣa,144 isolates belonged to SCCmecⅢ and 1 isolate was not genotyped by this method.Conclusion： SCCmecⅢ genotype was the major epidemic isolates in Xi′an.