摘要
目的:探讨滑膜细胞体外培养方法,了解黄板树根在骨性关节炎(osteoarthritis,OA)中的作用及意义。方法:取OA患者(n=22)关节滑膜组织,随机分为对照组(n=11)和干预组(n=11),分别原代培养滑膜细胞,采用酶联免疫吸附测定法(ELISA)定量检测两组滑膜细胞培养上清液中炎性因子IL-18蛋白的表达水平。结果:酶消化加组织块贴壁培养法可适用于体外培养试验用滑膜细胞;ELISA检测OA患者对照组细胞培养上清液中IL-18的含量为(61.324±19.281)pg/mL,干预组细胞培养上清液中IL-18的含量为(20.823±2.746)pg/mL,具备组间统计学差异(P<0.05)。结论:黄板树根可以使OA关节滑膜细胞中炎性因子IL-18表达下调,提示其可能对OA有治疗作用。
Objective：To explore the culture method in vitro about synoviocyte,to study the effect and significance of Huang Ban tree in osteoarthritis.Methods：Gathered the synovium from OA patients（n=22）,divided control group（n=11） and intervention group（n=11）,then take primary cell culture.The concentrations of IL-18 were measured in human OA synoviocyte culture fluid of two group by enzyme-linked immunosorbent assay（ELISA）.Results： Enzymatic digestion and tissue culture were refer to synoviocyte culture in vitro.The average value of IL-18 in control group was（61.324 ±19.281） pg/mL.The average value in intervention group was（20.823 ±2.746） pg/mL.There was a significant statistics difference between them（P0.05）.Conclusion：Huang Ban tree could decrease the expression of IL-18 in synoviocyte of OA.It may play an important therapeutical effect role in OA.
出处
《实用中西医结合临床》
2012年第2期1-3,共3页
Practical Clinical Journal of Integrated Traditional Chinese and Western Medicine
基金
国家自然科学基金资助项目(编号30571890)
广东省中药管理局课题(编号2007309)
