内皮素在大鼠急性胰腺炎发病中的作用

THE ROLE OF ENDOTHELIN IN THE PATHOGENIC MECHANISM OF ACUTE PANCREATITIS IN RATS

目的 了解内皮素对大鼠急性胰腺炎的影响。方法 应用蛙皮素及高分子右旋糖酐造成大鼠急性水肿性和出血坏死性胰腺炎模型 ,观察血浆内皮素水平及其它相关指标 ,同时观察外源性内皮素对水肿性胰腺炎大鼠血清淀粉酶、6 Keto PGF1α及胰腺组织病理形态等的影响。结果 出血坏死组大鼠血浆内皮素水平明显升高 ,与水肿组相比差异有显著性意义 (P <0 .0 1) ;一定剂量的外源性内皮素可促使水肿性胰腺炎向出血坏死性转化 ,胰腺组织损害程度与血浆内皮素水平呈正相关。结论 内皮素参予了急性胰腺炎的发生发展 ,并在水肿性胰腺炎向出血坏死性胰腺炎的转化过程中起重要作用。

Objective To study the effects of endothelin (ET) on acute pancreatitis in rats. Methods The acute edematous pancreatitis (AEP) and hemorrhagic necrotizing pancreatitis (AHNP) model of rats were induced by cerulein and dextran-110 and endothelin-1 was administered on AEP rats via intravenous injection. Serum amylase, plasma ET and 6-Keto-PGF 1α, pancreas hostologic observation were determined. Results The serum concentration of amylase increased markedly in AHNP rats, and there was a significant difference between AHNP and AEP (P<0.01). A dose of extrinsic ET-1 may induce the conversion of AEP to AHNP in rats. The degree of pancreatic damage correlates positively with the level of the plasma ET. Conclusion Endothelin might take part in the development of acute pancreatitis, and play a key role in the conversion of AEP to AHNP.