微波辅助提取猪皮胶原蛋白工艺优化

Optimization of Microwave-assisted Extraction Process for Collagen from Pigskin

为了保持胶原蛋白的生物活性、提高得率,采用微波技术提取猪皮胶原蛋白。通过单因素试验研究微波处理温度、微波处理时间、固液比、提取时间4个因素对胶原蛋白提取率的影响,确定微波辅助提取胶原蛋白的最佳工艺条件。结果表明:微波时间2min、微波温度25℃、固液比1:30(g/mL)、提取时间35h条件下,猪皮胶原蛋白的提取率为76.72%。

In order to maintain the bioactivity and improve the extraction rate of collagen,microwave technique was used to extract collagen from pigskin.Using one-factor-at-a-time combined with orthogonal array design method,the extraction rate of collagen was investigated with respect to temperature,microwave treatment time,material/liquid ratio and extraction time.The optimal extraction conditions were obtained as follows：microwave treatment time 2 min,temperature 25 ℃,material/liquid ratio 1：30（g/mL）,and extraction time 35 h.Under these conditions,the extraction rate of collagen from pigskin was up to 76.72%.