大孔吸附树脂纯化石蒜鳞茎中的加兰他敏

Purification of Galanthamine from Lycoris radiate Bulbs by Macroporous Resin Adsorption

目的:筛选出分离纯化石蒜中加兰他敏的最佳大孔树脂型号及工艺条件。方法:通过静态、动态相结合的方法,以加兰他敏吸附率、解吸率为指标,确定最佳工艺。结果:HPD300型树脂具有最佳的吸附和洗脱参数,其最佳工艺为粗提物上样液中加兰他敏1.74g/L、吸附流速2BV/h、最大上样量9BV;吸附后先以4BV水洗去杂质,再用8BV 70%乙醇溶液以2BV/h的速率进行洗脱,加兰他敏纯度可由原来的10.89%提高到45.52%。结论:HPD300可较好地吸附分离石蒜中加兰他敏,操作简单、安全、成本低廉,有较高的应用价值。

The best type of macroporoous resin and the best process conditions were screened for the purification of galanthamine from Lycoris radiate bulbs.Static and dynamic adsorption and desorption experiments were conducted to find out the effects of various process conditions on the adsorption and desorption rates of galanthamine.Macroporous resin type HPD 300 was found to be the best of 9 types investigated.The optimal purification process was obtained as follows：9 BV of crude extract containing 1.74 g/L galanthamine was loaded at a flow rate of 2 BV/h and then the HPD 300 column was washed with 4 BV of purified water for the removal of unwanted impurities before elution with 8 BV of 70% ethanol at a flow rate of 2 BV/h.By using the optimal purification process,the purity of galanthamine was increased from 10.89% to 45.52%.From these results,we came to the conclusion that HPD 300 allows better adsorption of galanthamine,which is easy to operate,safe and inexpensive,and therefore has a promising application potential.