微波预处理水酶法提取茶叶籽油工艺优化

Optimization of Microwave Pretreatment Followed by Aqueous Enzymatic Extraction of Tea Seed Oil

研究微波处理茶叶籽仁,水酶法提取茶叶籽油的工艺条件。茶叶籽仁粉碎60目加6倍质量的水,经过800W微波处理10min,加入纤维素酶1.5%、果胶酶2.0%、蛋白酶0.25%,采用pH4.5、酶解温度45℃、酶解6h,离心萃取茶叶籽油。结果表明,微波预处理茶叶籽能够促进水酶法提取茶叶籽油,出油率达27.9%。

The present paper describes an investigation into the extraction of tea seed oil by microwave pretreatment followed by aqueous enzymatic extraction.Tea seeds were pulverized to 60 mesh and added to a 6-fold volume of deionized water before microwave treatment for 10 min at a power level of 800 W.Thereafter cellulase,pectinase and acid protease were added at respective doses of 1.5%,2.0% and 0.25% for hydrolysis at pH 4.5 and 45 ℃ for 6 h.Finally centrifugation and extraction were sequentially conducted to obtain tea seed oil.Microwave pretreatment could promote the aqueous enzymatic extraction of tea seed oil,resulting in an extraction yield of 27.9%.