大鼠慢性肾功能不全模型的建立

Establishment of model in chronic renal insufficiency of rats

目的 研制一种特殊的大鼠慢性肾功能不全模型。方法 手术摘除右肾后 1周 ,重复 2次静注阿霉素 ,观察 2～12周时大鼠 2 4h尿蛋白排泄量和 3个月时的肾功能、病理组织学改变。结果 模型组大鼠除呈明显肾病综合征表现外 ,血尿素氮和肌酐水平较正常组明显增加 ,尿渗透压降低 ;病理学检查显示该组大鼠硬化肾小球增多 ,小球硬化指数增加 ,小管间质炎症细胞浸润 ,灶性小管萎缩。结论 右肾摘除和阿霉素注射均可导致肾损害 ,两者联合应用可在

Objective To establish a special animal model of chronic renal inefficiency (CRI) in Wistar rats. Methods At 1 week after right kidney of rats was removed by operation, some rats were received adriamycin (Adr) twice (5 and 3 mg·kg -1 , respectively) at 3 week intervals. All animals were divided into 4 groups: control, right kidney nephrectomy (RKX), Adr, and CRI. Urinary protein excretion (UPE) of 24 hours in all rats was observed at 2 to 12 weeks, and plasma biochemical parameters and histological examination were investigated at 3rd month. Results In the third month after Adr administration, besides the typical nephrotic syndrome, the levels of blood urea nitrogen and plasma creatinine were increased more ( P <0 01) in group CRI than that in control. The level of osmolality was remarkably decreased. Histologically, mesangial cellularity slightly increased and extracellular matrix significantly expanded in the majority of glomeruli. Tubules showed apparently focal atrophy and many proteincasts were found in dilated tubules. Moderate fibrosis in interstitium was also observed. Conclusion Renal injury may be induced by RKX or Adr administration, and CRI is produced in combination with RKX and Adr administration in three months.