法洛四联症患儿心肌细胞内钙运转与心功能关系的研究

Relationship between myocardial calcium transport and cardiac function of tetralogy of Fallot children

目的 探讨法洛四联症 (TOF)患儿心肌细胞钙运转与心功能的关系。方法 钙荧光指示剂Fura - 2法测定心肌细胞游离钙浓度 ,HP - 10 0 0型超声心动图机测定心功能。结果 2 8例TOF患儿红细胞游离钙和总钙浓度较健康组明显高 ,而钙泵活性明显低下 ;其中 8例舒张功能正常者红细胞钙泵活性与健康组无差别 ,而 2 0例舒张功能异常者不仅钙泵和钠泵活性较舒张功能正常者明显下降 ,且红细胞游离钙和总钙浓度亦较舒张功能正常者升高。结论 TOF患儿心肌细胞内钙运转与其心肌舒张功能异常密切相关。

Objective To explore the relationship between myocardial calcium transport and cardiac function of tetralogy of Fallot (TOF) children.Methods The concentrations of ionized calcium of the myocardium were measured by using calcium influorescence indicator Fura-2;the cardiac function of TOF children was determined by HP-1000 type ECHO .Results The concentrations of ionized and total calcium of erythrocyte increased significantly in 28 TOF children, but the activities of membrane calcium pump decreased markedly as compared with those of healthy groups.There was no obvious difference between the activities of membrane pump of healthy groups and 8 patients with normal diastolic function .In 20 cases with abnormal diastolic function,the activities of membrane pump decreased significantly but the concentrations of ionized and total calcium of the erythrocyte increased significantly as compared with normal diastolic function.Conclusion There is close relationship between the disturbance of the calcium transport in myocardial cells and diastolic dysfunction which exists in TOF children.