中国水仙居群遗传多样性的ISSR分析

ISSR Analysis on Chinese Narcissus Group Genetic Diversity

本实验利用ISSR分子标记技术研究5个中国水仙（Narcissus tazetta var．chinensis Boem＆Chen）居群的遗传多样性。从40个随机引物中筛选出8个有效引物，共扩增出69个可分析位点，其中多态位点65个，多态性务带比率（PPL）为94．20％。实验表明基因分化系数（Gst）平均值为0．0622，居群间的基因流（Nm）为7．5378，上述结果表明中国水仙居群的遗传变异大部分存在于居群内部，5个居群间的基因交流很高，种群间的分化很少。

In this study, study on ISSR markers of five Chinese narcissus （Narcissus that tazetta var chinensis, the Boem ＆ Chen） population genetic diversity. The eight primers selected from 40 random primers amplified a total of 69 analyzed sites ofpolymorphic loci and 65, the percentage of polymorphic bands （PPL） is 94.20%. Experiments show that the average gene differentiation coefficient （Gst） was 0.0622, gene flow among populations （Nm） 7.5378, the above results show that Xianju group of Chinese water most of the genetic variation within populations, five genes among populations the exchange of high population differentiation.