HPLC测定静脉给药后大鼠血清中得力生注射液中酯蟾毒配基与柔红霉素的含量

High Performance Liquid Chromatography Determination of Serum after Intravenous Injection in Delisheng Toad Poison Ester Ligand and Daunorubicin Content

目的:建立大鼠血清中酯蟾毒配基与柔红霉素浓度的测定方法。方法:血清经乙酸乙酯提取,炔诺酮(酯蟾毒配基)、多柔比星(柔红霉素)作内标,采用高效液相色谱法测定酯蟾毒配基与柔红霉素浓度。色谱柱:phenomenex C18(250 mm×4.6 mm,5μm),流动相:甲醇-0.01 mol/L磷酸二氢铵溶液(pH 4.65)-冰醋酸(V∶V∶V=30∶20∶0.1)(柔红霉素),乙腈-水(55∶45)(酯蟾毒配基),检测波长:柔红霉素233 nm;酯蟾毒配基296 nm。结果:酯蟾毒配基在55.0～8800μg/L范围内,柔红霉素在0.1478～14.778 mg/L范围内,待测物与内标物的峰面积与浓度呈现良好线性关系,r=0.9997(酯蟾毒配基),r=0.9984(柔红霉素),平均回收率均>80%,日内、日间RSD均<5%(n=3)。结论:该方法快速、简便、准确,适合于临床得力生注射液与柔红霉素浓度的监测。

Objective： To establish the serum ester toad poison ligand and determination of the concentration of daunorubicin.Methods：The serum extracted with ethyl acetate,norethindrone（ester toad poison ligand）,doxorubicin（DNR） as internal standard,using high performance liquid chromatography with toad poison ligand ester soft red mold hormone doxorubicin（DNR） as internal standard,using high performance liquid chromatography with toad poison ligand ester soft red mold hormone.Concentrations： phenomenex C18（250mm × 4.6mm,5μm）,mobile phase： methanol-0.01mol /L ammonium dihydrogen phosphate solution（pH4.65）-acetic acid（V∶ V∶ V = 30∶ 20∶ 0.1）（DNR）,acetonitrile-water（55∶ 45）,（ester toad poison ligand）,detection wavelength∶ daunorubicin 233nm;ester toad poison ligand 296nm.Results： ester toad poison ligand in 55.0 ~ 8800 μg / L-1 within the DNR at 0.1478 ~ 14.778 mg / L-1 range,analyte and internal standard peak area and concentration showed good linearity relationship,r = 0.9997（ester toad poison ligand）,r = 0.9984（DNR）,the average recoveries were 80 %,in one day or between two days RSD were 5%（n= 3）.Conclusion： This method is fast,simple,accurate,suitable for clinical Delisheng injection and monitoring the concentration of daunorubicin.