冠心汤湿法粉碎制备汤剂与传统汤剂的五种成分含量比较

Contrast Research of Five Ingredients Content of Guanxin Decoction Using Shattering Extraction with that of Traditional Decoction

目的:比较冠心汤湿法粉碎制备与其传统汤剂及煎药机煎煮的汤剂中5种成分的含量差异。方法:采用高效液相色谱(HPLC)法,用乙腈-0.05%磷酸溶液梯度洗脱,同时测定红花黄色素A、芍药苷、阿魏酸、丹酚酸B、丹参酮ⅡA含量。结果:冠心汤湿法粉碎制备的汤剂中5种有效成分的含量大大高于传统法及煎药机煎煮的汤剂。结论:湿法粉碎制备汤剂,方便快速,提取液有效成分含量高,作为中药汤剂一种新的制备方法值得进一步研究。

Objective：To contrast of five ingredients content of the preparation of Guanxin decoction using the shattering extraction with that of traditional decoction.Methods：To measure the content of tanshinone ⅡA,salvianolic acid B,paeoniflorin,ferulate and hydroxysafflor yellow A of guanxin Decoction by HPLC gradient elution,mobile phase consisted of acetonitrile-water.Results：The five ingredients content of preparation of Guanxin decoction using the shattering extraction are much more than that of traditional decoction.Conclusion： The preparation of traditional decoction using the shattering extraction is efficient,saving time and simple.