青蒿素分子印迹聚合物分子识别性研究

Study on recognition properties of artemisinin molecular imprinted polymer

目的合成对青蒿素具有特异识别能力的分子印迹聚合物。方法采用分子印迹技术合成了以青蒿素为模板分子,丙烯酰胺为功能单体,乙二醇二甲基丙烯酸酯为交联剂的青蒿素分子印迹聚合物。运用平衡结合试验研究了聚合物的吸附特性和选择识别能力。结果 Scatchard模型分析表明,该分子印迹聚合物在识别青蒿素分子的过程中存在两类不同的结合位点。高亲和力结合位点的离解常数Kd1=1.87 mmol/L,最大表观结合常数QMAX1=27.99μmol/g;低亲和力结合位点的离解常数Kd2=48.5 mmol/L,最大表观结合常数QMAX2=334.4μmol/g。结论结合底物的实验结果表明,青蒿素分子印迹聚合物对青蒿素具有良好的特征吸附和选择识别性能,为天然产物中高效分离纯化活性成分青蒿素提供了一种新型材料。

Objective To prepare a molecular imprinted polymer with specific recognition ability for artemisinin.Methods A molecular imprinted polymer was prepared by the molecular imprinting technique using artemisinin as the template molecular,acrylamide as the functional monomer,and ethylene glycol dimethacrylate as the cross-linking agent.The molecular imprinted polymer was investigated in equilibrium binding experiment to evaluate its adsorption property and selective recognition.Results Scatchard analysis showed that two classes of binding sites were formed in the artemisinin molecular imprinted polymer under the studied concentrations.The dissociation constant（Kd） and the apparent maximum binding constant（Qmax） were Kd1= 1.87 mmol/L,Qmax1= 27.99 μmol/g for high affinity binding sites and Kd2= 48.5 mmol/L,Qmax2= 334.4 μmol/g for low affinity binding sites,respectively.Conclusion The experiments of binding different substrates indicate that the imprinted polymer possess a high selectivity and a high recognition ability for artemisinin.It’s likely to be a novel material for separation and purification of active ingredients from natural products.