低剂量电离辐射对小鼠免疫器官Bcl-2蛋白表达的影响

Effectof low dose radiation on Bcl-2 protein expressions in mouse immune system

目的 :研究低剂量电离辐射对小鼠胸腺、脾和肠系膜淋巴结 Bcl- 2蛋白表达的影响 ,以揭示辐射诱导细胞凋亡 J型曲线的分子机理。方法 :采用免疫组织化学及图像分析定量方法。结果 :假照组各免疫器官存在 Bcl- 2蛋白的基础表达 ;而照射组随着照射后时间的推移 ,Bcl- 2蛋白表达逐渐增高 ,2 4 h达峰值 ,且差异显著 ;72 h回复至假照水平。结论 :上述结果为揭示低剂量辐射免疫兴奋效应和辐射诱导细胞凋亡

? Objective:In the present sutdy we observed the effect of dose radiation (LDR) on the Bcl- 2 protein expressions in mouse immune system.Methods:Immunohistochemistry and image analysis were used.Results:These results showed that Bcl- 2 protein had a basic expression in the sham- irradiated group. As the time passed,the expressions of Bcl- 2 protein gradually increased following whole- body irradiation with75m Gy X- rays.It reached the peak value at1 2～ 2 4 hours,and then returned to the basic level at 72 hours.Conclusion:These findings reveal the molecular mechanisms of the J- shape dose response curve occurring in apoptosis in the immune organs and the enhancementof the immune function after LDR. 〔