过敏性紫癜患者一氧化氮及肿瘤坏死因子的检测及临床意义

Detection of plasma nitric oxide and tumor necrotic factors in children with HSP and its clinical significance

目的 :探讨过敏性紫癜 (HSP)患儿一氧化氮 (NO)及肿瘤坏死因子 (TNF)的变化及其临床意义。方法 :采用改良的 Griess法、酶联免疫吸附试验 (ELISA)双抗体夹心法检测 32例 HSP患儿及 2 8例正常健康儿血浆中 NO及 TNF活性水平。结果 :HSP急性期血浆 NO及 TNF水平较正常对照组均明显升高 (P均 <0 .0 1 ) ,恢复期则接近正常 (P均 >0 .0 5)。急性期紫癜性肾炎 (HSPN)患儿血浆 NO及 TNF含量较同期单纯 HSP患儿血浆 NO及 TNF含量有所升高 ,但均达到显著差异。急性期 HSP患儿血浆 NO与 TNF呈显著正相关 (r=0 .835,P<0 .0 1 )。结论 :NO及 TNF均参与了

Objective:To explore the change of nitric oxdie(ON) and tumor necrotic factors(TNF) in children with Henoch- Schonlein purpura (HSP) and the clinical significance.Methods:The plasma NO and TNF in children (32 with HSP,2 8normal control) were detected with Griess,double- antibody sandwish ELISA.Results:The plasma levels of NO and TNF in patients of HSP (54.52 8± 1 6.674,0 .851± 0 .1 67) were significantly higher(P<0 .0 1 ) than those in the healthy group(40 .452± 1 2 .0 37,0 .346± 0 .0 4 6) . The plasma levels of NO and TNF in patients of Henoch- Schonlein purpura nephritis(HSPN) were higher butnot significantly higher than those in the HSP(P>0 .0 5) ,and there was a significantcorrelation(r= 0 .835,P<0 .0 1 ) between the plasma level of NO and TNF.Conclusion:The plasma NO and TNF might play a role in the pathogenesis and development of HSP children. 〔