正常早孕及反复流产妇女血清中TNFα、IL-6水平的检测

Determination of levelsoftumornecrosisfactor-alpha,interleukin6in serum of women with normal early pregnancy and recurrentabortion

目的 :探讨肿瘤坏死因子 (TNFα)、白细胞介素 6(IL- 6)与正常早孕、反复流产的关系。方法 :采用 ELISA方法对 30例正常非孕妇女、30例正常早孕妇女及 60例反复流产妇女血清中TNFα、IL - 6水平检测。结果 :正常早孕妇女血清中 TNFα明显低于正常非孕妇女 (P<0 .0 5) ,正常早孕妇女血清中 IL - 6水平与正常非孕妇女无明显差异 (P>0 .0 5) ,反复流产组血清中 TNFα、IL- 6明显高于正常早孕组 (P<0 .0 1 ) ,其中难免流产组明显高于先兆流产组 (P<0 .0 5)。结论 :提示正常早孕妇女 TNFα分泌受抑制 ,血清中 TNFα、IL- 6水平升高与反复流产有关 。

Objective:The purpose of the paper was to study the relation between cytokine,and normal early pregnancy and recurrent abortion.Methods:The levels of TNF -α,IL - 6 in serum from 30 nonpregnant women,30 women with normal early pregnancy and51 women with recurrent abortion were investigated by ELISA.Results:TNF- α levels of serum in normal early pregnant women(0 .2 0 3± 0 .0 95 ng/ml) were significantly lower that those in nonpregnant women(0 .368± 0 .1 2 3ng/ml) (P<0 .0 1 ) . There were no significant difference of IL- 6levels in serum between normal early pregnant women and nonpregnant women(P>0 .0 5) .TNF- α and IL- 6levels in recurrent abortion women(0 .61 1± 0 .558ng/ ml) were far higher than those in normal early pregnant women(P<0 .0 1 ) .And TNF- α and IL- 6levels in inevitable abortion women were higher than those in threatened abortion women.Conclusion:The release of TNF-α was inhibited in normal early pregnant women.Elevation of TNF-α and IL - 6levels of serum in recurrent women was one cause of inducing abortion.Measurements of the cytokine levels in serum may be a useful clinical marker to evaluate the resultof pregnancy. 〔