大鼠肾移植急性排斥模型建立研究

Establishment of acute renal rejection model in rats after kidney transplantation

目的建立大鼠肾移植急性排斥反应模型,探讨肾移植组织病理机制。方法 78只正常雄性SD大鼠分为正常对照组(A组)15只,模型组(B组)15只,非急性排斥组(C组)16只(以SD大鼠为供体),急性排斥组(D组)16只(以Wistar大鼠为供体),观察术后第7天各组SD大鼠肾功能变化,术后第7天切取移植肾,光镜下观察组织病理改变,参照Banff 97诊断标准,进行急性排斥反应严重程度的半定量评分。结果 C,D组尿素氮、肌酐水平及急性排斥反应半定量评分明显高于A组(P<0.05),D组明显高于C组(P<0.01)。结论 Wistar-SD大鼠移植模型可作为大鼠肾移植急性排斥模型。

Objective To establish the model of acute renal rejection in rats after kidney transplantation and investigate the pathomechanism of transplanted tissue.Methods Seventy-eight normal male SD rats were divided into normal group（group A,n=15）,pseudo-surgery group（group B,n=15）,non-acute graft rejection group（group C,n=16,SD rats as donor）,and acute graft rejection group（group D,n=16,Wistar rats as donor）.The renal function of these four groups was evaluated at the seventh day after transplantation operation.Grafts were harvested at the seventh day and were observed the pathological changes under the light microscope.Acute rejection semiquantitative scores were analyzed according to the standard of Banff 97.Results The serum levels of urea nitrogen,creatinine and acute rejection semiquantitative scores were significantly higher in group C and group D than those in group A（P0.05）,and were higher in group D than those in group C（P0.01）.Conclusion Wistar-SD rats kidney transplantation can be used for acute rejection model after kidney transplantation.