罗氏E170全自动电化学发光免疫分析仪剩余试剂再利用结果分析

Roche E170 automatic electrochemiluminescence immunoassay remaining reagent recycle used result analysis

目的探讨Roche E170全自动电化学发光免疫分析仪剩余试剂合并再利用的可行性。方法采用合并剩余试剂分别检测特定血清中5个肿瘤标记物[血清甲胎蛋白(AFP)、癌胚抗原(CEA)、糖类抗原(CA199、CA153、CA125)]的含量,并进行精密度、准确性及回收率分析;与新试剂分别检测同一组标本中5个项目低、中、高3个浓度的含量,并做均值比较及相关性分析。结果合并剩余试剂检测AFP、CEA、CA199、CA153、CA125 5个项目低、高浓度血清批内、批间变异系数(CV)均符合相关标准。质控血清准确性在允许范围内,回收率均值在90%～110%之间。用合并剩余试剂与新试剂分别检测5个项目低、中、高3个浓度,各组均值比较差异无统计学意义(P>0.05)。结论 Roche E170免疫分析仪可以对剩余试剂合并再利用,能够保证检测质量,降低检测成本。

Objective To investigate the feasibility of recycle of remaining reagent in Roche E170 automatic electrochemiluminescence immunoassay.Methods Alpha fetoprotein（AFP）,carcino-embryonic antigen（CEA）,carbohydrate antigen（CA）199,CA153 and CA125 in specific sera were detected by remaining reagents and original reagents.The precision,accuracy and recovery rate of remaining reagents were analyzed.The mean values and correlation of the 5 items in 3 different concentrations of sera（low,middle and high levels） were compared by 2 kinds of reagents.Results The within-run and between-run coefficients of variation（CV） of the 5 items measured by remaining reagents in low and high levels of sera met the related standard.The accuracy was within the allowance range.The recovery rate was variable from 90% to 110%.Comparing the results of the original reagents with the remaining reagents,there was no statistical significance of 3 different levels of the 5 items（P0.5）.Conclusions The remaining reagent can be recycled by Roche E170 automatic electrochemiluminescence immunoassay,which also can ensure the quality of measurement and the reduction of the cost.