某地区丙型病毒性肝炎患者HCV基因分型研究

HCV genotype analysis in patients with viral hepatitis type C in certain area

目的了解该地区丙型病毒性肝炎(以下简称丙型肝炎)患者丙型肝炎病毒(HCV)基因分型情况。方法分别以ELISA和重组免疫印迹试进行血清标本抗HCV抗体筛查和确认,对抗HCV抗体阳性标本用实时荧光定量PCR进行病毒载量检测。对病毒载量大于103copy/mL的标本用多重PCR进行HCV基因分型。结果 125例抗HCV抗体阳性标本中,HCV基因型主要为1b、2a、3a、1c、2c的检出率分别为58.4%、21.6%、3.2%、4.0%和8.0%。结论该地区HCV感染主要以1b型为主,其次是2a和3a型,检出其他地区少见的1c和2c型,说明该地区丙型肝炎流行的基因型呈多样性。

Objective To investigate hepatitis C virus（HCV） genotypes in patients with viral hepatitis type C in certain area.Methods ELISA and immunoblotting assay were performed for the screening and confirmation of anti-HCV in serum samples,and the virus load in confirmed samples were detected by real-time quantitative PCR.Samples with virus load more than 103 copy/mL were analyzed for HCV genotypes by using multiplex PCR.Results Among 125 cases of samples,positive with anti-HCV,the detection rates of HCV genotype 1b,2a,3a,1c and 2c were 58.4%,21.6%,3.2%,4.0% and 8.0%.Conclusion The most common HCV genotype in patients with HCV infection was 1b,followed by 2a and 3a,and genotype 1c and 2c,rarely detected in other districts,were also could be detected,indicating that the geno types of hepatitis C in this area might be diverse.