Ca^(2+)-CaM系统对野生苦菜耐盐抗氧化系统的调控

Regulation of Ca^(2+)-CaM on Antioxidant System of Salt Tolerance in Sonchus oleraceus L.

用两种从不同位点阻断Ca2+信号途径的抑制剂,即Ca2+螯合剂EGTA(乙二醇双四乙酸)(5 mmol/L)和CaM拮抗剂W7(N-氨乙基-5-氯-1-磺胺酰萘)(0.3 mmol/L),分别处理盐胁迫(0.5 mmol/L)下的野生苦菜幼苗,测定其抗氧化系统中酶的活性,H2O2、Pro和可溶性蛋白的含量。结果表明,EGTA(5 mmol/L)、W7(0.3 mmol/L)可降低超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)的活性,提高H2O2的含量,降低Pro和可溶性蛋白的含量,说明Ca2+-CaM系统与野生苦菜耐盐性密切相关。

The seedling of Sonchus oleraceus L.were treated by two inhibitors（EGTA and W7） under salt stress condition（0.5 mmol/L）,and the enzyme activity,the contents of H2O2,Pro and soluble protein of the antioxidant system were determined.The results showed that,after treating by EGTA（0.5 mmol/L） and W7（0.3 mmol/L）,the SOD activity,POD activity,CAT activity and the contents of Pro and soluble protein decreased,with the H2O2 content increased,indicating a close relationship between Ca2＋-CaM system and the salt tolerance of S.oleranceus L.