肺栓塞患者外周血单个核细胞干扰素相关基因mRNA表达

The expression of interferon associated genes mRNA in patients with pulmonary embolism

目的分析肺栓塞（PE）患者外周血单个核细胞IFN相关基因mRNA表达。方法采用全人类基因组表达谱芯片检测PE组（20例PE患者）和对照组（20例对照者）受试者外周血单个核细胞（PBMC）IFN相关基因mRNA表达。结果与对照组比，PE组受试者PBMCI型IFN中IFNα5EmRNA、IFNα6mRNA、IFNα8mRNA、IFNα14mRNA、IFNKmRNA、IFNω1mRNA、IFNε1mRNA表达下调，差异有统计学意义（P〈0．05）；I型IFN受体IFNαR1，mRNA、IFNαR2mRNA表达差异无统计学意义（P〉0．05）；Ⅱ型IFN——IFNγmRNA表达下调，差异有统计学意义（P〈0．05）；IFNγ受体——IFNγR1mRNA、IFNγR1mRNA表达上调，差异有统计学意义（P〈0．05）。结论PE患者中I型和Ⅱ型IFN基因表达显著下调，提示IFN下降是分泌生成减少，结果导致PE患者对病毒、胞内菌、寄生虫易感。免疫功能降低在PE的病理生理中发挥重要作用。

Objective To investigate the gene expression difference of IFN and their receptors in peripheral blood mononuelear cells （ PBMC ） of pulmonary embolism （PE） patients. Methods Twenty cases of PE patients and twenty sex and age matched controls were recruited into the study. Human cDNA microarray analysis was used to detect the gene expression difference of IFN associated genes between the two groups, and random variance model corrected t test was used to analyze the statistical data. Results In comparison with the control group, mRNA expression of type I IFN, including IFNα mRNA, IFNα6 mRNA, IFNαs mRNA, IFNα14 mRNA, IFNK mRNA, IFNα1 mRNA, IFNε1 mRNA in PBMC of PE patients were down-regulated （ P 〈 0. 05 ）. There was no significant difference in gene expression of type I IFN receptors IFNαR1 and IFNαR2 between the PE and control groups （ P 〉 0. 05 ）. In comparison with the control group, mRNA expression of IFNγ gene was down-regulated （ P 〈 0. 05 ）. The mRNA expression of IFNγR1 and IFNγR2 genes were upregulated compared with the control （P 〉 0. 05）. Conclusion mRNA expression of type I and type II IFN in PE are significantly down-regulated, but not the IFN receptors. Reduced immune function may play an important role in the PE patients who are susceptible to virus, intracellular bacteria and parasites.