急性肺损伤大鼠肝X受体表达的变化

Alteration of LXR Expression in Rats Lung With Acute Injury

目的观察急性肺损伤(ALI)大鼠肺组织内肝X受体(LXRs)的表达情况,探讨其在ALI炎症调控过程中的可能作用。方法将50只雄性SD大鼠随机分为5组:对照组、LPS处理后1h组、2h组、4h组和8h组。用LPS静脉注射复制大鼠ALI模型,分别在LPS处理1h、2h、4h、8h后处死大鼠,用RT-PCR与Western印迹检测LXRs表达水平,同时测定动脉血气和肺组织湿/干比值(W/D weight ratio)。结果与对照组小鼠相比,LPS处理组大鼠肺组织W/D较对照组均显著升高;动脉血氧饱和度均显著降低;LXRα/β mRNA的表达在LPS处理2 h时开始下降,在2h、4h和8h时差异有显著性;而LXRα/β蛋白的表达在LPS处理4h时开始下降,在4h和8h时差异有显著性。结论脂多糖(LPS)所致的ALI大鼠肺组织内LXRs表达显著减少,表明LXRs可能参与了ALI炎症反应的调控。

Objective To investigate the expression and potential role of liver X receptors（LXRs）in lung of rats with acute lung injury induced by lipopolysaccharide（LPS）.Methods 50 male SD rats were divided randomly into five groups：NS（normal saline）control group,LPS injured after 1 h group（LPS 1 h）,LPS injured after 2 h group（LPS 2 h）,LPS injured after 4 h group（LPS 4 h）and LPS injured after 8 h group（LPS 8 h）.Semi-quantitative reverse transcription polymerase（RT-PCR） and western-blot were used to measure expression of LXRs.The ratio of lung wet-dry weight（W/D） and arterial blood gas analysis were measured respectively.Results Compared to the control group,the ratio of lung wet/dry weight was significantly increased in LPS injured groups,the PaO2 was significantly decreased.The expression of LXRα/β mRNA begins to decrease at 2 h after LPS injured and significantly decreased at 4h and 8h group,While the expression of LXRα/β protein begins to decrease at 4 h after LPS injured and significantly decreased at 8h group.Conclusion The expression of LXRs in acute lung injury（ALI） of rats induced by LPS significantly decreased.The data suggested that LXRs might involve in the regulation of inflammation in ALI.