敌百虫联合高脂饲料喂养大鼠动脉粥样硬化模型的建立

Establishment of the Rat Models of Atherosclerosis Using Trichlorfon and High-fat Diet

目的探索建立类似人类长期接触低剂量、低毒性有机磷酸酯农药诱导动脉粥样硬化(As)大鼠模型的方法。方法健康SD大鼠,雌雄各半,随机分为普食喂养的对照组及敌百虫腹腔注射的高脂饲料喂养的模型组。结果第4、8周后模型组大鼠血清总胆固醇(TC)、高密度脂蛋白胆固醇(HDLC)、低密度脂蛋白胆固醇LDLC及As指数(AI)均显著高于对照组(P<0.05);HDLC/TC比值均显著低于对照组(P<0.01);主动脉弓及肝脏形态学观察发现,模型组出现明显的As病变。结论本实验初步建立了As大鼠模型,可作为研究人类长期接触低剂量、低毒性有机磷农药引起的大血管病变及其药物治疗的动物模型。

Objective To establish an effective method of setting up an animal atherosclerosis model that simulates the human atherosclerosis disease after long-term exposure to low doses of low toxic organic phosphate pesticide.Methods Healthy Sprague Dawley rats,half male and half female,were divided randomly into two groups equally：normal dieted group,high-cholesterol dieted and injected intraperitoneally with a low-dose Trichlorfon（HCHL＋Trichlorfon） group.TC,TG,HDLC and LDLC in serum were detected 0,4 and 8 weeks from the start.Then the rats were killed separately and their arteries were isolated and fixed in 10% formalin for HE staining.Aortic histomorphology changes were observed by HE staining.Results On the 4th,8th-week after treatment,the levels of TC,HDLC,LDL-C and the value of AI in HCHL＋Tichlorfon group were significantly higher than those in normal diet group（P0.05）,but the ratio of HDLC and TC were significantly lower in groups with HCHL＋Tichlorfon than those in groups with normal diet（P0.05）.The damages of the aortas in normal diet group were lesss severe than that in model group.So the successful model was made.Conclusion An atherosclerosis model of rats can be successfully established with Trichlorfon plus high-cholesterol diet,this study provides the animal model for investigating human atherosclerosis disease after long-term exposure to low doses of low toxic organic phosphate pesticide.