摘要
目的比较MTT法和EdU标记法两种常用测定细胞增殖的方法的优劣。方法以化疗药物顺铂浓度梯度处理前列腺癌细胞株PC-3,分别采用MTT法和EdU标记技术进行细胞增殖变化测定。结果 MTT组顺铂浓度增至5μg/mL时细胞活力和增殖明显受抑(P<0.05);EdU掺入组顺铂浓度增至2μg/mL时增殖细胞阳性率和胞内荧光强度均显著下降(P<0.05)。结论 MTT法简单、快速、经济,结果与活细胞数有关,间接反映细胞的增殖状态;EdU标记技术灵敏、客观,特异性标记分裂期细胞,直接反映细胞的增殖。
Objective To compare the detection sensitivity between MrlT and EdU incorporation for cell proliferation assay. Methods Prostate cancer cell line （PC-3） was exposed to different concentrations of cisplatin for 24 hours. Cells viability and proliferation were respectively detected by MTI＂ and EdU incorporation assay. Result PC-3 cell viability and proliferation were significantly decreased in 5 μg/mL condition by MTT detection （ P 〈 0.05 ）, however, the proliferation positive cell ratio and the density of fluorescence were significantly decreased in 2μg/mL condition by EdU incorporation assay （ P 〈 0.05 ）. Conclusion Simple, fast and economic are the advantages of MTI＂ method, but it only reflects the sta- tus of viable cells, independent with proliferation population; sensitivity, objectivity and specificity are the advantages of EdU incorporation assay, which is related with S phase cell cycle, directly reflects the cells proliferation.
出处
《山东医药》
CAS
2012年第12期70-72,I0003,共4页
Shandong Medical Journal
基金
北京协和医学院青年基金(imc-2010)
公益性科研院所基本科研业务费专项基金(imc-2008)
