泥鳅激素浸泡催产试验的研究

A Preliminary Report on Exogenous Hormones Soaking Experiment of Loach

为了寻找一种简易的泥鳅催产方法。浸泡催产激素有绒毛膜促性腺激素(HCG)、促黄体素释放激素类似物(LHRH-A2)和地欧酮(DOM),以HCG250IU+LHRH-A22μg+DOM1mg/尾为基本剂量(X),食盐作为浸泡助渗剂,食用色拉油为消泡剂。分别进行不同浸泡时间、浓度和盐度的泥鳅催产试验。累计激素浸泡催产种鳅13.987kg,900尾,获受精卵80.218万粒,其催产率在0%～100%,平均催产率为34.66%;得泥鳅水花苗71.42万尾。孵化率为在45.6%～93.3%之间,孵化率平均值为81.21%。激素浸泡催产率高达80.0%,激素浸泡催产获得泥鳅受精卵的孵化率和注射激素催产获受精卵的孵化率差别不大,说明利用激素浸泡泥鳅催产方便省时,是一种有前途的新方法。

The objective of this study was to find a simple method to induce spawning.Exogenous hormones in this experiment included HCG and LHRH-A 2 and DOM.Every loach was in accordance with basic amount of HCG250 IU and LHRH-A 2 2 μg and DOM1 mg.Salt was used as aided agent for soaking and salad oil was used to eliminate foam.We studied the effect of different soaking time and different concentrations and different salinity on propagation of loach.The total amount of loach for soaking experiment was 13.987 kg and 900 loaches were injected into exogenous hormones and 802.18 thousand spawning eggs were collected.The rate of inducing spawning was from 0%-100%.The average spawning rate was 34.66% and 714.2 thousand loach juveniles were got and the hatching rate was from 45.6%-93.3%.The average value of hatching rate was 81.21%.The rate of induced spawning induced by exogenous hormones was 80% and there was no significant difference on hatching rate between injection hormones and hormones soaking method.This study indicatedthat hormones soaking method was likely a new convenient,time-saving and promising method for loach.