摩西球囊霉优良繁殖基质的筛选

Screening of the Superior Culture Substrates for Glomous mosseae

为获大量摩西球囊霉(Glomous mosseae,简称G.m)的繁殖体,以高丹草(Sudangrass hyrid L.)为宿主植株接种G.m,盆栽培养12周,研究6种基质处理对G.m的繁殖参数和高丹草的生长参数的影响,以及基质理化性质、脲酶、蔗糖酶和酸性磷酸酶的活性变化。结果发现,基质Ⅵ处理后G.m的繁殖参数和生长参数均高于其他处理,其碱解氮、速效磷、速效钾、pH和有机质含量分别为121.5 mg/kg,13.47 mg/kg,106.8 mg/kg,6.9和32.3%。相关性分析显示,G.m的定殖率与菌根侵染率和孢子密度呈正相关,与接种前基质的碱解氮、速效钾和有机质含量呈负相关,与速效磷和pH呈正相关;与收获后基质的碱解氮、速效磷、速效钾、有机质、脲酶、蔗糖酶和酸性磷酸酶活性呈负相关,与pH呈正相关。侵染率和孢子密度与接种前基质理化指标呈正相关,与速效磷的正相关系数最高,其次是碱解氮;侵染率和孢子密度与收获后基质的碱解氮、速效钾、pH、有机质和酸性磷酸酶活性呈正相关,与脲酶和蔗糖酶活性呈负相关。G.m的优良繁殖基质为草炭土:田间土:河沙(体积比2:2:1)。

To get more the vegetative forms of G.m strains,Sudangrass hyrid L.,as the host plants were inoculated Glomous mosseae（G.m） 12 weeks with 6 different proportions of culture mediums in pot culture,the influence of the growth parameters of the host plants and breeding parameter of G.m were studied and the influence of physical and chemical properties of mediums and the activity of urease,invertase,acid phosphatase were analyzed.The results showed that,the growth parameters of the host plants and breeding parameter of G.m after treatment with medium Ⅵ were higher than others treatments,the content of alkali-hydrolyzed nitrogen（N）,available phosphorus（P）,available potassium（K）,pH,organic matter was 121.5 mg/kg,13.47 mg/kg,106.8 mg/kg,6.9,32.3% respectively.Correlation analysis showed the colonization rate was positively correlated with infection rate,spore density and which was negatively correlated with alkali-hydrolyzed N,available K,organic matter and was positively correlated with available P,pH of pre-inoculated,meanwhile it was negatively correlated with alkali-hydrolyzed N,available P,available K,organic matter and the activity of urease,invertase acid phosphatase of after-harvest mediums.Infection rate and spore density which were positively correlated with the index of physical and chemical properties,the