灰葡萄孢霉高效拮抗木霉菌株的筛选及其翻译延伸因子序列分析

The Screen of the Antagonism of Trichoderma spp. Against Botrytis cinerea and Sequence Analysis of Translation Elongation Factor

为进一步开发利用木霉菌资源,对从菜田土壤中初步分离纯化获得的12株木霉菌株,采用对峙培养和生长速度测定法进行灰葡萄孢霉(Botrytis cinerea)高效拮抗木霉菌株的筛选,并通过翻译延伸因子序列同源性比较对其进行分子鉴定。结果表明:菌株Tr 9701和Tr 1108的生长速度快,对病原菌的抑制率高,且协同应用有一定的增效作用。经对Tr9701和Tr1108翻译延伸因子同源序列分析,并结合其形态特征结果表明,Tr9701为绿色木霉(Trichoderma viride)和Tr1108为深绿木霉(Trichoderma atroviride)。绿色木霉和深绿木霉为菜田生境习居菌,2种菌株协同利用对蔬菜灰霉病有较好的协同增效作用,应进一步设计利用多靶位木霉菌来提高防病效果。

The aim was to utilize the biological agents of Trichoderma spp..There were 12 strains of Trichoderma species separated from vegetable fields,New antagonistic Trichoderma spp.were screened through the confront culture and growth rate determination,and the sequence homology of translation elongation factor was analyzed.The results showed that,strain Tr 9701 and Tr 1108 had high growth rates and strong inhibitions.With the identification of translation elongation factor of these strains,strain Tr9701 was classified as Trichoderma viride,and Tr1108 was classified as Trichoderma atroviride respectively.Trichoderma viride and Trichoderma atroviride were all inhabitants in vegetable field.The two strains which were used to control vegetable gray mold cooperatively were effective,so it should be studied to utilize Trichoderma spp.with more targets in order to increase control efficiency.