Maspin和VEGF-C蛋白表达与卵巢癌发生及临床病理学的关系

Expression of Maspin and VEGF-C protein in human ovarian carcinoma and its relationship with clinicopathological variables

目的研究Maspin和VEGF-C蛋白表达与卵巢癌的发生和临床病理因素的相关性。方法应用免疫组织化学技术检测60例卵巢癌(卵巢乳头状浆液性癌33例、卵巢子宫内膜样癌7例、卵巢黏液性癌20例)、18例良性上皮性卵巢肿瘤、14例交界性卵巢肿瘤标本及10例正常卵巢组织中Maspin和VEGF-C的蛋白表达,并进行对照比较。结果 Maspin和VEGF-C在卵巢癌中的阳性表达率分别为65.0%和68.3%,与卵巢交界性肿瘤、良性肿瘤、正常卵巢组织相比较,差异具有统计学意义(P<0.05);卵巢癌组织中,Maspin的表达与病理类型、分化程度无相关性(r=0.125,P=0.34;r=-0.037,P=0.778),与FIGO临床分期、淋巴结转移、腹水密切相关(r=-0.364,P=0.004;r=0.311,P=0.015;r=-0.292,P=0.024)。VEGF-C的表达与淋巴结转移、FIGO临床分期、腹水有相关性(r=-0.280,P=0.031;r=0.377,P=0.003;r=0.384,P=0.002)。Maspin与VEGF-C蛋白表达呈负相关(r=-0.349,P=0.006)。结论卵巢癌细胞中Maspin和VEGF-C蛋白均有较高水平表达,可能在卵巢癌的血管发生、进展、淋巴结转移中发挥着重要作用。联合检测卵巢癌中Maspin和VEGF-C蛋白的表达,有利于更好地评估卵巢癌的生物学行为,提高对患者预后判断的准确性。

Objective To investigate the expression and correlation between Maspin,VEGF-C and the tumor genesis of the ovary,clinical-pathological variables.Methods Using immunohistochemistry,Maspin and VEGF-C expression of 60 ovarian carcinoma tissues（33 serous papillary carcinomas,7 endometrioid carcinomas,and 20 ovarian mucinous carcinomas） and 18 benign ovarian tumors,14 borderline ovarian tumors and 10 normal ovarian tissues were examined and compared.Results The positive expression rates of Maspin and VEGF-C were 65.0%and 68.3%respectively,there were significant difference between these expression in tissues of ovarian carcinoma and borderline carcinoma,benign tumor,normal ovarian tissues;over expression of Maspin was not correlated with pathological type and the degree of differentiation（r =0.125,P = 0.34;t = -0.037,P =0.778）,but it was correlated with International Federation of Gynecology and Obstetrics（FIGO） stage,presence of metastatic lymph nodes,and the presence of ascites（r = -0.364,P =0.004;r =0.311,P =0.015; r = - 0,292,P = 0.024）.Over expression of VEGF-C was correlated with presence of metastatic lymph nodes,FIGO stage, and the presence of ascites（r = -0.280,P =0.031;r =0.377,P =0.003;r =0.384,P = 0.002）.Maspin was negatively correlated with VEGF-C（r = -0.349,P =0.006）.Conclusion Over expression of Maspin and VEGF-C was detected in ovarian carcinoma.High expression of Maspin and VEGF-C are seemed to play a role in ovarian cancer angiogenesis, progression and lymph node metastases.Combination detection the expressions of Maspin and VEGF-C is helpful for evaluating the biological characteristics and predicting the prognosis of ovarian carcinoma.