呋喃C-30对变异链球菌生物膜早期形成的影响

Effect of furanone C-30 on initial biofilm formation of Streptococcus mutans

目的:探讨密度感应拮抗剂呋喃C-30对变异链球菌生物膜早期形成的影响。方法:将体外合成的密度感应拮抗剂呋喃C-30按终浓度10、100μmol/L分别配制于含变异链球菌的牛心脑浸液培养基,37℃微需氧培养24h,形成生物膜后,用生物膜定量分析仪检测生物膜形成的量。结果:100μmol/L呋喃C-30组变异链球菌生物膜的形成受到显著抑制,磁珠成像开始减弱和完全消失的时间均迟于对照组;生物膜开始形成后,其生物膜形成指数低于对照组,差异有统计学意义(P<0.05)。结论:呋喃C-30在100μmol/L浓度时能有效抑制变异链球菌生物膜的形成,其应用可能为龋病防治提供新的思路。

Objective：To research the effect of quorum sensing inhibitor furanone C-30 on initial biofilm formation of Streptococcus mutans.Methods：The in vitro synthetic quorum sensing inhibitor furanone C-30 was prepared with BHI medium in which Streptococcus mutans was cultivated into different final concentration of 10μmol/L,100μmol/L.Biofilms were formed after being incubaed at 37℃ for 24 h,followed which the kinetic quantification of biofilm formation was detected by BioFilm Ring Test.Results：The furanone C-30 group with a concentration of 100μmol/L was shown to inhibit the biofilm formation by Streptococcus mutans significantly（P0.05）.The time of magnetic beads immobilized and the value of BFI were both different between the 100μmol/L furanone C-30 group and the control groups.Conclusions：Quorum sensing inhibitor furanone C-30 in the concentration of 100μmol/L can inhibit the biofilm formation of Streptococcus mutans.It may direct a new way for the prevention and therapy of caries.