麦穗鱼的组型分析和DNA含量测定

Karyotypic analysis and cellular DNA contents of Pseudorasbora parva

采用PHA和秋水仙素活体注射、肾细胞短期培养和空气干燥制片法,对麦穗鱼染色体数目和核型进行研究;以麦穗鱼外周血细胞为样本,鸡血细胞DNA为标准(2.30pg),使用美国贝克曼库尔特公司Cell LabQuanta SC流式细胞仪测定了麦穗鱼二倍体细胞的DNA含量。结果显示:麦穗鱼染色体数目2n=50,未观察到次缢痕及性染色体,亦未发现有随体,核型公式为18m+22sm+10st,NF=90;麦穗鱼二倍体细胞DNA含量为鸡血对照的1.38倍,绝对含量为3.18pg。

The chromosome number and karyotype of Pseudorasbora parva were analyzed using a routine method including intraperitoneal injection of PHA and colchicine,cultivation in vivo,and slides preparation with air-drying.Whilst erythrocyte nuclear DNA content of P.parva was determined by using a flow cytometer（Beckman Coulter Inc.,USA） with serving the chicken erythrocytes（their DNA content is 2.30 pg） as reference cells.Results obtained in this study showed that：the diploid chromosome number of P.parva was 50（2n=50）;the karyotype of this species was 2n=18 m＋22 sm＋10 st,NF=90,while the secondary constriction,heterosome and satellite chromosome were not observed;the diploid cellular DNA contents of P.parva was 3.18 pg,which was 1.38 times to the chicken erythrocytes＇.