摘要
目的研究T细胞免疫球蛋白及黏蛋白结构域分子3(Tim-3)在胃癌组织中的表达及其临床意义。方法采用流式细胞术检测52例胃癌及15例胃炎患者手术标本Tim-3的表达。结果 CD4+和CD8+T细胞均可见Tim-3的表达,胃癌组织CD4+T细胞Tim-3表达为(12.4±8.41)%,明显高于胃炎组的(6.01±1.95)%;胃癌组织CD8+T细胞中Tim-3表达为(9.56±7.48)%,明显高于胃炎组的(5.39±2.98)%,差异均有统计学意义(P<0.05)。Tim-3在CD4+T细胞中的表达与胃癌患者的浸润深度、淋巴结转移和TNM分期有关(P<0.05),而与性别、年龄、肿瘤大小、组织学类型均无关(P>0.05)。Tim-3在CD8+T细胞中的表达仅与胃癌患者的TNM分期有关(P<0.05),而与其他临床病理学特征均无关(P>0.05)。结论Tim-3在胃癌的侵袭、转移过程中可能起着重要作用,是胃癌患者的不良预后因素之一。
Objective To evaluate the expression of Tim-3 and its clinical significance in the gastric cancer tissues.Methods Fifty-two gastric cancer specimens and 15 gastritis tissues were detected for Tim-3 by flow cytometry,and 15 specimens of gastritis tissues served as the control.Results Tim-3 expressed both in gastric cancer tissues and gastritis tissues.In CD4＋T cells,the expression level of Tim-3 in gastric cancer tissues（12.4±8.41）% was significantly higher than gastritis tissues（6.01±1.95）%;In CD8＋T cells,the expression level of Tim-3 in gastric cancer tissues（9.56±7.48）% was significantly higher than gastritis tissues（5.39±2.98）%（P0.05）.Tim-3 expression in CD4＋T cells was significantly associated with clinicopathological features such as invasion depth,lymph node metastasis and TNM stage（P0.05）.No correlation was found between Tim-3 and gender,age,tumor size and histological type（P0.05）.In CD8＋T cells,Tim-3 expression correlated with TNM stage rather than other clinicopathological features.Conclusion Tim-3 played an important role in the invasion and metastasis of gastric cancer,and was a prognostic factor for poor outcome.
出处
《临床肿瘤学杂志》
CAS
2012年第3期219-222,共4页
Chinese Clinical Oncology
基金
国家自然科学基金资助项目(81171653
30950022
30972703)
江苏省自然科学基金资助项目(BK2011246
BK2011247)
六大人才高峰第六批资金资助项目(BRA2010037)
江苏省卫生厅医学科技发展基金资助项目(P200932
P200933)
