外周血Th17细胞及IL-17^+FoxP3^+T细胞在非小细胞肺癌患者的表达变化

Distribution of peripheral blood T helper 17 cells and IL-17^+FoxP3^+T cells in patients with non-small-cell lung cancer

目的:探讨辅助性T 17(Th17)细胞及白细胞介素17(IL-17)+叉头蛋白3(FoxP3)+T细胞在非小细胞肺癌患者外周血中的表达及意义。方法:57例非小细胞肺癌患者作为研究对象,25名正常人作为对照。采用流式细胞术检测外周血Th17细胞、调节性T细胞(Treg细胞)的百分率以及IL-17+FoxP3+T细胞占Treg细胞的百分率。结果:Ⅳ期非小细胞肺癌患者外周血单核细胞(PBMC)中Th17细胞百分率高于Ⅰ~Ⅲ期患者及正常对照(均P<0.01)。非小细胞肺癌患者PBMC中的Treg细胞百分率高于正常对照,并且IL-17+FoxP3+T细胞占Treg细胞的百分率高于正常对照(P<0.05)。结论:Ⅳ期非小细胞肺癌患者中Th17细胞以及IL-17+FoxP3+T细胞数量增加,Th17以及IL-17+FoxP3+T细胞可能参与了非小细胞肺癌的肿瘤远处转移过程,从而影响肿瘤进程。

Objective To investigate the distribution of T helper 17（Th17） and interleukin-17（IL-17） forkhead box P3（FoxP3）T cells in peripheral blood lymphocytes in patients with non-small cell lung cancer （NSCLC） and its significance. Methods T ceils in peripheral blood of 30 patients with stage 1V and 27 patients with stage I -HI NSCLC were analyzed, and 25 healthy volunteers were served as controls. The percentages of Thl7 cell and regulatory T cell （Treg cell） in peripheral blood and percentage of CD4IL17FoxP3 T cell in Treg cells were determined by flow cytometry. Results The percentage of blood Th17 cell in peripheral blood mononuclear cells （PBMC） was significantly higher in patients with stage 1V NSCLC patients than in stage I -111 NSCLC patients and healthy controls （P〈0.05）. The percentage of Treg cell in PBMC and the percentage of CD4IL17FoxP3＊ T cell in Treg cells were significantly higher in patients with NSCLC than in healthy controls （P〈0.05）. Conclusions The percentages of Thl7 cell and CD4＋IL17FoxP3 cell were increased in patients with stage 1V NSCLC , which indicated that Thl7 cells and CD4IL17FoxP3 T cells might be involved in distant metastasis of NSCLC and thereby influencing the course of NSCLC. Key words： Non-small cell lung cancer; T helper 17 cells; Regulatory T cells