高良姜挥发油对不同肿瘤细胞株增殖的影响

Anti-proliferative activity of volatile oils extracted from Alpinia officinarum Hance on various carcinoma cell lines

目的评估高良姜挥发油对不同肿瘤细胞株的增殖抑制作用。方法利用水蒸气蒸馏法提取高良姜根茎的挥发油成分,采用MTT法观察其对人肝癌HepG2细胞、人结肠癌HT29细胞、人鼻咽癌CNE-2Z细胞、人甲状腺癌SW579细胞及人宫颈癌Hela细胞的增殖抑制率及半数抑制浓度(IC50)。结果高良姜挥发油提取率达1.2%,其可呈剂量依赖性抑制HepG2、HT29、CNE-2Z、SW579、Hela细胞株增殖,对HT29、Hela、CNE-2Z、HepG2、SW579细胞株的IC50值分别为2 352、717、458、431、329μg/mL。结论高良姜挥发油成分对多种类型肿瘤细胞的增殖均有一定抑制作用。

Objective To evaluate the inhibitory effect of volatile oils extracted from Alpinia officinarum Hance on proliferation of various carcinoma cell lines in vitro.Methods The volatile oils of Alpinia officinarum Hance was extracted by steam distillation,the proliferations and half inhibition concentration（IC50） were observed by MTT assay in hepatocellular carcinoma cell HepG2,colorectal carcinoma cell HT29,nasopharyngeal carcinoma cell CNE-2Z,thyroid carcinoma SW579 and cervical carcinoma cell Hela,which treated with different concentrations of volatile oils.Results The extraction efficiency of volatile oils extracted from Alpinia officinarum Hance was 1.2%,and the volatile oils inhibited the proliferation of HepG2,HT29,CNE-2Z,SW579 and Hela cells in concentration-dependent manner.The IC50 values of volatile oil against HT29,Hela,CNE-2Z,HepG2 and SW579 cells were 2 352,717,458,431,329 μg/mL respectively.Conclusion The volatile oil components from Alpinia officinarum Hance can inhibit the proliferation of various kinds of carcinoma cell lines to a certain degree.