幼鼠大脑GFAP阳性细胞具有多向分化潜能

Early Postnatal GFAP-expressing Cells Produce Multilineage Progeny in Mouse Cerebrum

目的:探讨幼鼠大脑胶质纤维酸性蛋白(GFAP)阳性细胞的分化类型。方法:hGfapCreERT2/Rosa26R转基因小鼠在出生后第6天用他莫西芬诱导Cre重组酶表达;在出生后第60天,对大脑组织切片行X-gal染色,然后用细胞种类特异性抗体Blbp、GFAP(星形胶质细胞标记物),NeuN(神经元标记物),NG2(少突胶质细胞前体细胞标记物)或CC1(成熟少突胶质细胞标记物)进行免疫组织化学染色。结果:大脑不同脑区X-gal染色阳性细胞可以和Blbp和GFAP、NeuN及NG2或CC1共标记。结论:出生后早期小鼠大脑GFAP阳性的细胞具有多向分化潜能,可分化产生星形胶质细胞、神经元和少突胶质细胞前体细胞。

Objective： To investigate the differentiation of early postnatal GFAP-positive cells in mouse brain.Methods： Tamoxifen injection induced Cre recombination expression in mice carrying hGfapCreERT2/Rosa26R transgence at P6.Cells expressing Cre recombination were indicated by X-gal staining and then immunohistochemical staining（Blbp,GFAP,NeuN and NG2 or CC1） was used to identify the cell style at P60.Results： X-gal staining positive cells coexpressed astroglial markers Blbp and GFAP,oligodendrocyte precursor cell marker NG2 or oli-godendrocyte cell marker CC1,and the neuronal marker NeuN,in different regions of the adult mouse brain.Conclusion： The early postnatal GFAP-positive cells exhibit properties of multiple differentiation potentiality,to eventually differentiate into neurons,astrocytes,and oligodendrocyte precursor cell in adult mice brain.