肝素对脓毒症大鼠肺损伤的影响

Effects of heparin on lung injury in sepsis rats

目的研究肝素对内毒素(LPS)诱导的脓毒症大鼠肺损伤的影响及血清组织因子途径抑制物(TFPI)含量的变化。方法将健康、雄性Wistar大鼠24只,随机分为正常对照组、LPS 2 h组、LPS+肝素2 h组,每组8只。尾静脉注射LPS建立脓毒症模型。于不同时间点处理大鼠后获得肺部组织,光镜下观察肺组织病理变化,进行比较;同时检测血清TFPI水平的变化。结果与正常对照组相比,LPS 2 h组和LPS+肝素2 h组血清TFPI均显著升高(分别为P<0.05,P<0.01);LPS+肝素2 h组与LPS 2 h组相比血清TFPI升高,差异有统计学意义(P<0.05)。病理观察发现正常对照组大鼠肺组织结构清晰,肝素干预组大鼠肺组织病理损害较LPS 2 h组减轻。结论肝素的应用可使脓毒症大鼠血清TFPI水平升高,显著改善大鼠肺组织的损伤。

Objective To explore the effects of heparin on the endotoxin-induced lung injury and serum levels of tissue factor pathway inhibitor（TFPI） in sepsis rats.Methods A total of 24 male Wistar rats were randomly assigned into 3 groups：normal control group（n=8）,lipopolysaccharide（LPS） 2 h group（n=8）,LPS＋heparin 2 h group（n=8）.Sepsis rat models were induced by intravenous injection of LPS.Levels of serum TFPI was detected at the different time.The pathological changes of lung tissues were observed under microscope after HE staining.Results TFPI was higher in LPS 2 h group and LPS＋heparin 2 h group than that in normal control group（P0.05 or P0.01）.Compared with LPS 2 h group,TFPI increased in LPS＋heparin 2 h group（P0.05）.Pathological results showed that the structure of lung tissues was clear in normal control group,and the damage of lung tissue was less in LPS＋heparin 2 h group than that in LPS 2 h group.Conclusion Heparin could markedly increase serum TFPI level and ameliorate the lung injury.