迷迭香叶片愈伤组织诱导及再分化培养

Callus Induction and Plant Regeneration from Rosemary Leaves

本研究以迷迭香叶片为外植体,探索愈伤组织形成及再分化条件。结果表明:蔗糖含量较高的培养基可促进愈伤组织形成,其中以MS+蔗糖50g/L+6-BA0.5mg/L+NAA0.5mg/L效果最好,诱导率可达88%;愈伤组织再分化形成不定芽时,以MS+6-BA1.5mg/L+KT0.5mg/L+NAA0.5mg/L效果较好,再分化率达50%;MS+6-BA0.8mg/L+NAA0.5mg/L诱导不定芽增殖,增殖率可达到300%多;不定芽生根时,MS+NAA0.1mg/L效果较好,生根率可达65%。同时,研究发现,尽管外植体被消毒至无菌,但75%乙醇复合其它灭菌剂共同灭菌时,会导致外植体大量死亡。

Rosemary leaves were used as explants in the study to investigate factors affecting callus induction and plant regeneration. It was found that the culture medium with higher concentration of sucrose promoted callusinduction. MS medium supplemented with 6-BA 0.5 mg/L, NAA 0.5 mg/L, and sucrose 50 g/L showed the best result with an induction rate of 88.8%. When regenerated callus develops shoots, it was found that MS mediumcontaining 6-BA 1.5 mg/L, KT 0.5 mg/L and NAA 0.5 mg/L showed relative good result with 50% regeneration rate. When grown in 6-BA 0.8 mg/L and NAA 0.5 mg/L, the amount of growth was increased by more than 300%.MS medium containing NAA 0.1 mg/L showed better result in inducing roots with rooting rate reaching 65%. Additionally, the study also showed that even though the explants were sterile, solution containing 75% ethanol with other sterilizing reagents would result in mass explant deaths.