期刊文献+

玉米Mutator转座子突变体库研究进展 被引量:3

Overview of Maize Mutant Libraries Mediated by Mutator Transposon
下载PDF
导出
摘要 Mutator转座子以其高的正向突变率、倾向插入基因富含区和低拷贝序列区等独特的遗传特性,使其在玉米基因功能的研究以及突变体库的构建中发挥了重要的作用。本文从群体大小、群体优缺点以及利用途径等方面对Mutator介导的玉米突变体库作了详细的介绍,以期能为我国玉米基础研究工作者提供启示和借鉴。 Mutator (Mu) transposon plays an important role in the research of both maize forward and reverse genetics. It was widely used to obtain transposon-tagging mutants due to its distinctive properties of high rates of forward mutation, preferential insertions in gene-rich and low-repetitive genomic regions. In this paper, we reviewed the scales, advantages, disadvantages and applications of each of these published Mu-tagging maize mutant stocks in the world in detail, which might provide a revelatory ideas and references for Chinese basic researchers in maize.
作者 李见坤 郑军
出处 《分子植物育种》 CAS CSCD 北大核心 2012年第2期238-244,共7页 Molecular Plant Breeding
基金 转基因生物新品种培育重大专项(编号:2009ZX08009-019B)资助
关键词 玉米 Mu转座子 突变体 突变体库 Maize (gea Mays L.), Mu transposon, Mutant, Mutant library
  • 相关文献

参考文献38

  • 1Asakura Y,Hirohashi T,Kikuchi S,Belcher S Osborne E Yano S Terashima I Barkan A and Nakai M. Maize mutants lacking chloroplast FtsY exhibit pleiotropic defects in the biogenesis of thylakoid membranes[J].Plant Cell,2004,(01):201-214.doi:10.1105/tpc.014787.
  • 2Bensen R.J,Johal G.S,Crane V.C,Tossberg J.T Schnable P.S Meeley R.B and Briggs S.P. Cloning and characterization of the maize An1 gene[J].Plant Cell,1995,(01):75-84.
  • 3Brutnell T.P. Transposon tagging in maize[J].Functional & Integrative Genomics,2002,(1-2):4-12.doi:10.1007/s10142-001-0044-0.
  • 4Cresse A.D,Hulbert S.H,Brown W.E,Lucas J.R and Bennetzen J.L. Mu1-related transposable elements of maize preferentially insert into low copy number DNA[J].Genetics,1995,(01):315-324.
  • 5Das L,Martienssen R. Site-selected transposon mutagenesis at the hcf106 locus in maize[J].Plant Cell,1995,(03):287-294.
  • 6Dietrich C.R,Cui F,Packila M.L,Li J Ashlock D.A Nikolau B.J and Schnable P.S. Maize Mu transposons are targeted to the 5' untranslated region of the gl8 gene and Sequeences flanking Mu target-site duplications exhibit nonrandom nucleotide composition throughout the genome[J].Genetics,2002,(02):697-716.
  • 7Edwards D,Coghill J,Barley J,Holdsworth M and Edwards K.J. Amplification and detection of transposon insertion flanking sequences using fluorescent muAFLP[J].Biotechniques,2002,(05):1090-109210941096-1097.
  • 8Femandes J,Dong Q.F,Schneider B,Morrow D.J Nan G.L Brendel V and Walbot V. Genome-wide mutagenesis of Zea mays L.using RescueMu transposons[J].Genome Biology,2004,(10):R82.doi:10.1186/gb-2004-5-10-r82.
  • 9Frey M,Stettner C,Gierl A. A general method for gene isolation in tagging approaches:amplification of insertion mutagenised sites (AIMS)[J].Plant Journal,1998,(05):717-721.doi:10.1046/j.1365-313X.1998.00091.x.
  • 10Gao M,Wanat J,Stinard P.S,James M.J and Myers A.M. Characterization of dull1 a maize gene coding for a novel starch synthase[J].Plant Cell,1998,(03):399-412.

同被引文献14

引证文献3

二级引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部