食管鳞癌患者肿瘤组织及血清中E-钙黏蛋白表达的临床意义

Expression of E-cadherin in the tumor tissue and serum of patients with esophageal squamous cell carcinoma

目的:检测食管鳞癌患者肿瘤组织及血清中E-钙黏蛋白(E-cadherin,E-cad)的表达并探讨其临床意义。方法:采用免疫组织化学法对48例食管鳞癌、23例糜烂性食管炎、24例正常食管黏膜组织中E-cad的表达进行检测;同时采用酶联免疫吸附法(ELISA)对上述研究对象血清中可溶性E-cad(sE-cad)的表达进行检测,并且收集完整的临床病理资料。结果:E-cad表达水平在食管鳞癌组织中明显降低,与正常食管黏膜上皮组织及糜烂性食管炎黏膜上皮组织相比,差异有统计学意义(P<0.05)。不同浸润深度、有无淋巴结转移及不同分化程度的食管鳞癌,其癌组织中E-cad的表达水平差异有统计学意义(P<0.05)。血清sE-cad在食管鳞癌组表达水平明显高于正常食管组及糜烂性食管炎组(P<0.05)。血清sE-cad水平与食管鳞癌的临床病理特征无关。食管鳞癌患者肿瘤组织中E-cad高表达者与低表达者相比,血清sE-cad水平差异无统计学意义(P=0.134)。结论:E-cad可作为食管鳞癌诊断及预后判断的辅助指标。血清sE-cad可作为食管鳞癌诊断筛查的血清学辅助指标。

Objective： To survey E-cadherin （E-cad） expression in tumor tissue and serum of esophageal squamous cell carcinoma patients, and to observe the clinical significance of their expression. Methods： Forty-eight samples of esophageal squamous cell carcinoma tissue, 23 samples of erosive esophagitis tissue, 24 samples of normal esophagus tissue and the corresponding sera were obtained. We used immunohistochemistry （IHC） to detect expression of E-cad in the tissues and enzyme-linked immunosorbent assay （ELISA） to examine expression of E-cad in the serum. Furthermore, we collected complete clinicopathological data from the participating patients.Results： q-he expression level of E-cad in the esophageal squamous cell carcinoma tissue was lower than that in normal esophagus tissues and erosive esophagitis tissues （P〈O.05）. Moreover, the expression level of E-cad was related to the depth of invasion, the status of lymph node metastasis and the level of differentiation of esophageal squamous cell carcinoma （P〈0.05）. The expression level of serum E-cad of esophageal squamous cell carcinoma patients was obviously higher than that in the serum of normal esophagus controls and erosive esophagitis patients （P〈0.05）. But the expression level of E-Cad in the serum of esophageal squamous cell carcinoma patients was unrelated to clinicopathological features. The expression level of E-cad in the tissue was not correlated with that in the serum（P=0.134）. Conclusion： The expression of E-cad in tissues may assistin the diagnosis and prognosis of esophageal squamous cell carcinoma. The expression of E-cad in the serum may assistin the diagnostic screening of esophageal squamous cell carcinoma.